In our preceding article, we explored the fundamentals of the Morris Water Maze (MWM) and some essential analysis techniques. In this article, we will investigate more sophisticated analysis methods that offer a deeper understanding of rodents’ spatial learning and memory.

One such advanced technique is the examination of search strategies. By studying the routes taken by rodents during the MWM task, scientists can classify the strategies employed into various categories, such as random search, chaining, or direct swims (Garthe et al., 2009). This can help establish whether an experimental intervention affects not only the performance but also the underlying tactics used by the animals.

Another method involves quadrant occupancy analysis, where the time spent in each quadrant of the pool is measured. A preference for the target quadrant (where the platform is situated) indicates improved spatial memory (Vorhees & Williams, 2006). This method can be especially beneficial during probe trials when the platform is removed, and the animal’s search behavior reflects its memory of the platform’s location.

Additionally, heatmaps and trajectory plots serve as valuable visualization tools, enabling researchers to discern patterns and trends in the animals’ spatial behavior. The HVS Image video tracking system allows scientists to generate these visual representations of MWM data (Gallagher et al., 1993).

References: Garthe, A., Huang, Z., Kaczmarek, L., Filipkowski, R. K., & Kempermann, G. (2009). Not all water mazes are created equal: Cyclin D2 knockout mice with constitutive hippocampal adult neurogenesis show specific pattern of search strategies in two water maze tests